Developing rapid generation technology involving wild lentil crosses in order to produce Aphanomyces-resistant lentil varieties – proof of concept

  • Lentils
  • Disease Management
  • Root Rots
  • University of Saskatchewan

Lead Investigator(s)

Dr. Sabine Banniza

Lead Investigator(s) Institution

University of Saskatchewan - CDC

Objective

To develop a modified rapid generation technique for wild and interspecific lentil germplasm that will enable the rapid development of adapted lentil germplasm with new traits such as aphanomyces root rot resistance.

Outcome

Crosses were made between var. Eston and a wild relative (L. ervoides L01-827A). The F2 population (LR-59) developed from this cross was then screened for root rot tolerance. Some LR-59 lines scored lower than both parents. Using a single seed descent method, the F2 population from LR-59 was carried forward until the 6th generation to fix desired traits and eliminate deleterious recessive alleles. A combination of optimum growing conditions for lentil resulted in a generation length of 60 days on average per cycle compared to 100 days under field or greenhouse conditions, as a result, six generations of lentil can be produced in one year. The developed rapid generation technology (RGT) method is simple, non-sterile, cost-effective, and can be applied to cultivated and wild lentil as well as segregating populations.

Genetics

SPG Contribution

$259,289.00

Project Status

Complete

Duration/Timeline of Project (Year to Year)

2015 - 2017