Development of Improved Markers for Mycosphaerella Blight Resistance in Pea
- University of Saskatchewan
Dr. Tom Warkentin
Lead Investigator(s) Institution
University of Saskatchewan - CDC
To identify quantitative trait loci (QTL), develop heterogeneous inbred families (HIFs), and then fine map QTLs segregating for mycosphaerella blight resistance to identify closely linked markers and/or candidate genes.
A recombinant inbred line (RIL) population developed from a cross between Alfetta (Pisum sativum) and P651 (P. fulvum) was evaluated for disease resistance and was genotyped using 1536 Single Nucleotide Polymorphisms (SNP) Illumina Golden Gate array. A high density genetic linkage map consisting of 733 SNP markers covering a genetic distance of 682.1 cM was used for quantitative trait loci (QTL) analysis. Six QTLs were identified for mycosphaerella blight under field and 3 additional QTLs were identified under greenhouse conditions. HIFs evaluated under field conditions in 2015 at Saskatoon showed a wide range of variation in disease scores and other traits. Genotyping of HIFs was conducted using 8 markers designed from an Illumina GoldenGate array.
Duration/Timeline of Project (Year to Year)
2013 - 2016
Saskatchewan Ministry of Agriculture - Agriculture Development Fund, Western Grains Research Foundation
Total Project Cost