Development of improved markers for mycosphaerella blight resistance in pea

  • Peas
  • University of Saskatchewan

Lead Investigator(s)

Dr. Tom Warkentin

Lead Investigator(s) Institution

University of Saskatchewan - CDC

Objective

To identify quantitative trait loci (QTL), develop heterogeneous inbred families (HIFs), and then fine map QTLs segregating for mycosphaerella blight resistance to identify closely linked markers and/or candidate genes.

Outcome

A recombinant inbred line (RIL) population developed from a cross between Alfetta (Pisum sativum) and P651 (P. fulvum) was evaluated for disease resistance and was genotyped using 1536 Single Nucleotide Polymorphisms (SNP) Illumina Golden Gate array. A high density genetic linkage map consisting of 733 SNP markers covering a genetic distance of 682.1 cM was used for quantitative trait loci (QTL) analysis. Six QTLs were identified for mycosphaerella blight under field and 3 additional QTLs were identified under greenhouse conditions. HIFs evaluated under field conditions in 2015 at Saskatoon showed a wide range of variation in disease scores and other traits. Genotyping of HIFs was conducted using 8 markers designed from an Illumina GoldenGate array.

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Genetics

SPG Contribution

$185,150.00

Project Status

Complete

Duration/Timeline of Project (Year to Year)

2013 - 2016

Co-funders

Saskatchewan Ministry of Agriculture - Agriculture Development Fund, Western Grains Research Foundation

Total Project Cost

$356,882.00