50K Single Nucleotide Polymorphisms (SNP) chip development for genomic-enabling chickpea breeding
- University of Saskatchewan
Dr. Bunyamin Tar'an
Lead Investigator(s) Institution
University of Saskatchewan - CDC
To develop tools to facilitate high throughput genome screening of key traits in chickpea.
The use of the DNA chip developed in this project in the analyses of five recombinant inbred populations showed on average of 24% polymorphic rate between the two parents in each population. Quantitative trait loci (QTL) analyses using the genetic maps developed based on the current SNP chip identified a suite of genetic loci associated with phenology and other agronomic traits in chickpea. Further application on diversity panel in a genome wide association study identified loci associated with seed protein and oil content in chickpea. The use of the current DNA chip would help in selection targeting seed protein and oil content in chickpea. Moderate accuracy was obtained when the current DNA chip was used in genomic selection (GS) for grain yield and seed quality. In conclusion a Cicer 60K SNP chip that greatly enhances genetic study and molecular breeding in chickpea has been developed.
Duration/Timeline of Project (Year to Year)
2017 - 2021