Towards generating multiple-fungal disease resistance in lentil
- Disease Management
- University of Saskatchewan
Dr. Sabine Banniza
Lead Investigator(s) Institution
University of Saskatchewan - CDC
To identify and characterize fungal disease resistance and virulance target genes from Lens ervoides (Le) involved in recognizing and triggering immune responses against pathogens of the three most economically important diseases of cultivated lentil: anthracnose, ascochyta blight and stemphylium blight.
The population was genetically characterized which allowed us to develop a genetic map that we used as a guide to locations in the genome that are associated with disease resistance. The population was also screened for resistance to all three diseases, which allowed us to identify eight locations on the genetic map that are associated with anthracnose resistance and three locations associated with stemphylium blight resistance. These locations are hot spots for resistance gene candidates, so with the help of genome information generated earlier in Dr. Bett’s group, we could catalogue all the genes located at these hot spots. It was not possible to use the genetic map to locate hot spots for resistance gene candidates for ascochyta blight, so we used two other approaches to identify genes or their products involved in resistance. In the first approach, we monitored the expression of genes specifically activated or suppressed in resistant compared to susceptible lines after their infection with the respective pathogen. In the second approach, rather than looking at gene expression, we monitored what proteins (the product of expressed genes) were synthesized and in what quantities in the wild lentil lines after challenge with those pathogens. We have prioritized 10 candidate genes for resistance to ascochyta blight, 27 for anthracnose and 20 for stemphylium blight resistance for further validation and testing as molecular markers in the breeding program.
Duration/Timeline of Project (Year to Year)
2014 - 2019
Saskatchewan Ministry of Agriculture - Agriculture Development Fund, Western Grains Research Foundation, Natural Sciences and Engineering Research Council of Canada
Total Project Cost