• Chickpeas
• Lentils
• Peas

Objective

To determine the hormone profiles necessary for rescue media for culturing chickpea embryos for interspecific hybrid development. To determine the hormone profiles necessary for chickpea breeding through doubled haploid technology.

Outcome

Using measurement of plant hormones in 4-, 8- and 12-day old seeds from pea, chickpea, and lentils showed that early embryo development stages in these legume species appears to be regulated by phytohormones. This information will be used for subsequent alteration of the current tissue culture media to provide and/or induce synthesis of needed phytohormones for the development of embryos from interspecific crosses.